https://www.ithaca.edu/academics/school-humanities-and-sciences/chemist…
Guest Speaker Professor Mikail Abbasov Ph.D. of Cornell University will be joining us on Tuesday, September 20th at 12:10 pm will present:
A Proteome-Wide Atlas of Lysine-Reactive Chemistry
Recent advances in chemical proteomics have begun to characterize the reactivity and ligandability of lysines on a global scale. Yet, only a limited diversity of aminophilic electrophiles have been evaluated for interactions with the lysine proteome. Recently, we have reported an in-depth profiling of >30 uncharted aminophilic chemotypes that greatly expanded the content of ligandable lysines in human proteins. Aminophilic electrophiles showed disparate proteomic reactivities that range from selective interactions with a handful of lysines to, for a set of dicarboxaldehyde fragments, remarkably broad engagement of the covalent small-molecule–lysine interactions captured by the entire library. We used these latter ‘scout’ electrophiles to efficiently map ligandable lysines in primary human immune cells under stimulatory conditions. Finally, we showed that aminophilic compounds perturb diverse biochemical functions through site-selective modification of lysines in proteins, including protein–RNA interactions implicated in innate immune responses. These findings support the broad potential of covalent chemistry for targeting functional lysines in the human proteome.